TOXICOLOGICAL EFFECTS OF ORGANOPHOSPHATE PESTICIDE, PHORATE ON THE PROSTOMIAL REGION OF EARTHWORM, METAPHIRE POSTHUMA
Suneel Kumar* and Satyendra M. Singh
Earthworm Toxicology Unit, Earthworm Ecology and Environmental Research Laboratory, Department of Animal Science, Mahatma Jyotiba Phule Rohilkhand University, Bareilly-243006 (Uttar Pradesh), INDIA.
Corresponding author E-mail*: firstname.lastname@example.org
Received: 02-03-2019 Revised: 08-05-2019 Accepted: 31-05-2019
Pesticides are known to produce morphological, anatomical and physiological changes in the vital organs of non targeted animals like earthworms which are considered as biomarkers of soil system. Phorate – a common organophosphate pesticide used in different agro ecosystems for the pest control also have adverse effect on the earthworms. The toxicity on the prostomial region was studied by 1/3rd of LC50 value of phorate for the duration of 30 days and histopathological observations were recorded. The experimental worms were exposed to the calculated sub-lethal dose of phorate for 30 days and histopathological studies of prostomial region were performed using routine paraffin method (Humason, 1979). The microscopic observations of histopathological manifestations of exposed Metaphire posthuma to phorate showed in the damage of prostomial epithelial tissues, necrosis of glandular cells and partial damage with cloudy swelling of longitudinal muscles, damage in circular and longitudinal muscles. It may be add concluded that toxic effect of phorate may change the structural integrity of the tissues that might be applied in the environmental risk assessment. Further, it may be added that imbalanced uses of pesticide in agricultural land may disturb the sustainability of biotic and abiotic parameters of the soil, which are solely responsible for the crop yield.
Keywords: Earthworm, Phorate, Histopathology, Metaphire posthuma, nts.