THE COMBINED USE OF CHEMICAL FERTILIZERS WITH MULTI –TRAIT PSEUDOMONAS FLUORESCENS TO ENHANCE BELL PEPPER GROWTH

THE COMBINED USE OF CHEMICAL FERTILIZERS WITH MULTI –TRAIT PSEUDOMONAS FLUORESCENS TO ENHANCE BELL PEPPER GROWTH
Anurag Yadav1*, Kusum Yadav2 and Shelly Gupta3
1*Department of Microbiology, College of Basic Science & Humanities, S.D. Agricultural University, S.K. Nagar, Gujarat
2Department of Biochemistry, University of Lucknow, Lucknow, Uttar Pradesh
3Department of Microbiology, Division of Life Sciences, S.B.S. (PG) Institute of Biomedical Sciences and Research,
Balawala, Dehradun
[Corresponding author E-mail1*: anuragyadav123@gmail.com]
Received: 25-07-2015 Accepted: 06-11-2015
Abstract:
An experiment was conducted at the S.B.S. (PG) Institute of Biomedical Sciences and Research, Uttarakhand, India to study the effect of co-treatment of Pseudomonas fluorescens Tf5 with five different chemical fertilizers on bell pepper (Capsicum annuum var annuum L., California wonder) plants. The experiment was based on complete randomized block design pattern with five replications. The rhizosphere of fenugreek (Trigonella foenum-graecum L.) plant was screened for efficient plant growth promoting fluorescent pseudomonad biovars. Of the total 139 bacterial isolates with fluorescent colonies, ten biovars of P. fluorescens were identified. These isolates were screened for plant growth promoting properties. Growth promoting traits like IAA production, phosphate solubilization and ammonia solubilization were used in screening. The most efficient biovar (P. fluorescens Tf5) was selected to check its effect on bell pepper growth. Treatments of sodium nitrate, diammonium phosphate, potash, superphosphate and urea with or without P. fluorescens Tf5 treatment were provided to bell pepper plants. Results show that with chemical fertilizer treatment the highest (72.28 cm) and the lowest (33.07 cm) plant length (shoot and root) was obtained from Diammonium phosphate (DAP) and control, respectively. The highest (5.68 g) and lowest (2.53 g) plant dry weigh was recorded with DAP and control respectively. Application of P. fluorescens Tf5 along with chemical fertilizers significantly enhanced plant height fresh, weight and dry weight. In a separate experiment spectrophotometric analysis showed that enhancing the fertilizer concentration negatively affects P. fluorescens Tf5 growth. Maximum bacterial growth retardation, in the form of optical density (OD) drop, was observed with urea treatment.

Keywords: Pseudomonas fluorescens, chemical fertilizers, Capsicum annuum, block design, optical density, weight, bacteria

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