PREVALENCE OF HEAVY METAL RESISTANT BACTERIA IN SAMPLES FROM SEWAGE TREATMENT PLANTS AND COMMON EFFLUENTS TREATMENT PLANTS

PREVALENCE OF HEAVY METAL RESISTANT BACTERIA IN SAMPLES FROM SEWAGE TREATMENT PLANTS AND COMMON EFFLUENTS TREATMENT PLANTS

Katyal*1, P. P. Sahota1, D. Singh2 and R. Sikka2
1Department of Microbiology, Punjab Agricultural University, Ludhiana-141001.
2Department of Soil Sciences, Punjab Agricultural University, Ludhiana-141001.
[Corresponding author E-mail*1: drpkatyal@pau.edu]
Received: 14-07-2015 Accepted: 05-11-2015
Abstract:
A total of twenty one morphologically distinct bacterial strains were isolated from selected-28 samples from sewage treatment plants (STPs) and one untreated sample from common effluent treatment plant located in Ludhiana district. Tolerance of the isolates against six heavy metals: Ni, Hg, As, W, Cr and Pb were tested. The isolated strains were characterised phenotypically. Samples procured from CETP (common effluent treatment plant) was also tested for the elemental profile by using Inductively coupled Argon Plasma-Atomic Emission Spectroscopy (ICAP-AES) and data revealed a very high level of Cu (52.91 ppm) and Ni (152 ppm). Detailed analysis of bacterial isolates in heavy metals contaminated sites revealed the presence of a variety of pigmented strains. Morphological characterization of the isolates have shown the prevalence of a number of Gram -ve strains which after biochemical characterization has been found to belong to genera Proteus, Vibrio, Klebsiella and Enterobacter as revealed by their biochemical analysis.

Keywords: bacteria, heavy metals, sewage, effluents, Argon Plasma-Atomic Emission Spectroscopy

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