IN SITU SCREENING OF PLANT GROWTH PROMOTING BACILLUS SUBTILIS AND PSEUDOMONAS FLUORESCENS

IN SITU SCREENING OF PLANT GROWTH PROMOTING BACILLUS SUBTILIS AND PSEUDOMONAS FLUORESCENS FROM CUMIN (CUMINUM CYMINUM L.) RHIZOSPHERE

Anurag Yadav* and Kusum Yadav
*Dept. of Microbiology, College of Basic Science & Humanities, S.D. Agricultural University, S.K. Nagar, Gujarat – 385506
Dept. of Biochemistry, University of Lucknow, Lucknow, Uttar Prasesh – 226012
*Corresponding author: anuragyadav123@gmail.com
Received: 25-02-2014 Accepted: 27-03-2014

Cumin rhizosphere was screened for isolating some plant growth promoting rhizobacteria. Bacillus subtilis and Pseudomonas fluorescens were specifically targeted in the screening. The rhizosphere isolates were studied morphologically and biochemically and identified as biovars of P. fluorescens and B. subtilis. The biovars were tested in situ for plant growth promoting traits by employing tests like indole acetic acid (IAA) production, NH3 production, HCN production, phosphatase activity and siderophore production. Quantitative estimation of IAA production and phosphate solubilization was also performed. The in vitro results of the tests proposed the use of three multi-trait biovars of P. fluorescens (PCU1, PCU4, and PCU17) and one of B. subtilis (BCU5) in cumin rhizosphere to test their plant growth promoting activity.

Keywords: Cumin, rhizosphere, Bacillus subtilis , Pseudomonas fluorescens, biovars, IAA, PCU1, PCU4, PCU17

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