EVALUATION OF CYTOTOXICITY OF MIRABILIS JALAPA WITH ALLIUM TEST

EVALUATION OF CYTOTOXICITY OF MIRABILIS JALAPA WITH ALLIUM TEST

Archana Srivastava
Department of Botany, D.G. (P.G) College, Kanpur
[E-mail: archanasrivastavadg@gmail.com]
Received: 18-07-2015 Accepted:24-10-2015
Abstract:
The cytotoxicity and genotoxicity of aqueous leaf extracts of Mirabilis were evaluated with the aid of the Allium test. The extract was found to exhibit mitotoxic and chromatotoxic effects. Plant extract decreased the mitotic index at all concentration and also induced nuclear lesion. Leaf extract mitotic index decreased to 3.08% (4mg) and 3.23 (4mg), respectively as compared to control 8.43% which showed more than 50% mitotic inhibition in Allium cepa. Whereas, decrease in 0.78% (2mg) and 0.84% (4mg) active mitotic index frequency has been recorded in extract of leaf extract as compared to control 2.28%. The abnormalities observed included nuclear lesions, binucleate cells, prophase accumulation otherwise called prophase poison, sticky metaphase, anaphase bridge. No abnormal cells were observed at telophase. The implications of these anomalies were also discussed. The results lend more support to the reports of several investigators on the urgent need to standardize the dosages of medicinal plant extracts for the treatment of various ailments. The results of this study suggest that, although mirabilis has beneficial effects as a medicinal herb, it can cause serious problems and damage on cells when used improperly.

Keywords: Cytotoxicity , genotoxicity, Mirabilis, Allium test, mitotic index

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