EFFICACY OF LAWSONIA INERMIS LEAVES EXTRACT AGAINST COPPER (CU++) TOXICITY INDUCED IN FISH CHANNA PUNCTATUS (BLOCH.)

EFFICACY OF LAWSONIA INERMIS LEAVES EXTRACT AGAINST COPPER (CU++) TOXICITY INDUCED IN FISH CHANNA PUNCTATUS (BLOCH.): A STUDY BASED ON CHROMOSOMAL ABERRATIONS

Vivek Kumar and Sunil P. Trivedi*
Environmental Toxicology & Bioremediation Laboratory (ETBL),
*Department of Zoology, University of Lucknow, Lucknow- 226007
[email : vivekkumarsb@gmail.com; Corresponding aurhor* email : sat060523@gmail.com]
Received: 04-03-2015 Revised: 21-05-2015 Accepted: 28-05-2015
Abstract:
Lawsonia inermis, commonly known as ‘Henna’ or ‘Mehndi’, is an evergreen, medium sized shrub of the family Lythraceae. The leaves paste of this plant has been traditionally used for dying hair, skin and nails. Besides, cosmaceutical usages, the plant also holds ample significance in traditional medicines. The present work is focused to screen the efficacy of ethanolic extract of henna leaves against copper (Cu++), a known mutagen in freshwater fish, Channa punctatus. In the experiment, fish were divided into six groups. Fish of group-1 served as control. Fish of group-2 and group-3 were exposed with 96 h-LC50/10 of Copper sulphate and 3 mg/l concentration of L. inermis, respectively. Fish of group 4, 5 and 6 were treated with 1 mg/l, 2 mg/l and 3 mg/l concentration of L. inermis along with 96h-LC50/10 of Copper sulphate. The chromosome was prepared from fish kidney collected at multiple intervals (24, 48, 72 and 96 h). Copper sulphate, in its tested concentration induces chromosomal aberration in group-2, in a time dependent manner. However, on simultaneous exposure of fish with different concentration of L. inermis (1, 2 and 3 mg/l) along with 96h-LC50/10 of copper sulphate reduces the frequency of chromosomal aberrations. This reduction in chromosomal aberrations on introduction of L. inermis in group-4, 5 and 6 establishes the excellent protective potential of this plant extract in fish, C. punctatus. Thus, present study opens new vistas for the conservation of fish biodiversity by employing indigenous traditional knowledge (ITK) in the form of “Henna”.

Keywords: Lawsonia inermis, cosmaceutical usages, Channa punctatus, toxicity, copper, conservation

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