EFFICACY OF INTRAVENOUS INOCULATION OF EVANS BLUE DYE (EBD) TO DIFFERENTIATE DEAD AND LIVE CHICKEN EMBRYO

EFFICACY OF INTRAVENOUS INOCULATION OF EVANS BLUE DYE (EBD) TO DIFFERENTIATE DEAD AND LIVE CHICKEN EMBRYO

Manesh Kumar, P.1*, Raja, P1 and Tembhurne, P. A.2
1 Department of Animal Biotechnology, Madras Veterinary College, Vepery, Chennai-600007;
2Department of Veterinary Microbiology and Animal Biotechnology Teaching & Research Cell, Nagpur Veterinary College, Seminary Hills, Nagpur-440006.
[Corresponding author E-mail1*: drmaneshkumar@rediffmail.com]
Received: 22-09-2015 Accepted: 02-10-2015
Abstract:

Evans blue dye is a potential intra-vital and nontoxic that binds with the albumin and used to determine its distribution pattern in the chicken embryos. We assessed the efficiency of intravenous inoculation (IV) with EBD in the chicken. The results suggest that the efficiency of IV inoculation found to be 70% in chicken embryos, the dead embryos were stained blue colour compared to live embryos harvested after 48 hrs post inoculation indicating the ability of the dye to stain the damaged and injured tissues. Inoculum volume and concentration of the EBD is pivotal as the intensity of blue colour in the dead embryo was higher in groups inoculated with 100 μl and 200 μl compared to the group inoculated with 50 μl of 1% EBD. Hence this technique can be used to assess EBD labelling and determine the distribution of the EBD in circulation at embryonic stages using chicken embryo as a model. This technique could also be employed prior to the intravenous inoculation of chicken embryos with oligonucleotides used in miRNA Knockdown studies, with drugs for testing is effect on embryo model or with viruses (BTV) to study its pathological effects on chicken embryo.

Keywords:  Efficacy, EBD, Chicken, Embryo, miRNA, BTV

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