Anindita Mali*, N. Karthikeyan and G. Kumaresan

Department of Livestock Products Technology-Dairy Science

Veterinary College and Research Institute, Namakkal-2, Tamil Nadu



Received:  13-06-2019                                                                                  Accepted: 21-12-2019

Cottage cheese is a high protein low fat cheese variety. The present study was to develop a synbiotic cottage cheese, using Lactobacillus acidophilus (LA-5) with various levels of pectin 0.1, 0.2, 0.3, 0.4 and 0.5 per cent, and selecting the optimum level by sensory evaluation. It was found that 0.3 per cent was the desirable level of pectin to be added. This synbiotic cottage cheese was subjected to physico-chemical and microbial analysis with commercial cheese starter culture. The synbiotic cottage cheese using Lactobacillus acidophilus (LA-5) and commercial cheese starter culture showed good microbial quality and almost similar probiotic viability. It is concluded that synbiotic cottage cheese incorporated with 0.3% pectin scored good overall acceptability and microbial quality with recommended levels of probiotic viability.

Key words: synbiotic cottage cheese, Lactobacillus acidophilus (LA-5), pectin


Cottage cheese is a soft, fresh cheese curd product with a mild flavour that is uncured and obtained from skim milk. The term originated from cheeses made by early Americans settlers in their small dwellings, in the 1830’s right on the stove. The cottage cheese shows an adequate profile to incorporate probiotic cells and prebiotic substances. In addition, it is a healthy alternative by virtue of its low-fat content. It is rich in proteins, relatively low in fat and high in calcium, phosphorous, sodium, selenium and vitamin B12.

A prebiotic is non-digestible food ingredient that pass undigested through the upper part of the gastrointestinal tract and stimulate the growth or activity of advantageous bacteria that colonize the large bowel by acting as substrate for them.

Pectin is a structural heteropolysaccharide contained in the primary cell walls of terrestrial plants. It is considered as a soluble dietary fibre and exerts physiological effects on the gastrointestinal tract, such as reducing glucose absorption, hypocholesterolaemic effect and delaying gastric emptying. In addition, the oligosaccharides obtained from pectin have been proposed as an excellent new generation prebiotic (Hotchkiss et al., 2003).

The world health organization (WHO, 2002) defines probiotics as live micro-organisms that “when administered in adequate amounts, confer a health benefit on the host.”

Lactobacillus acidophilus is one of the most common type of probiotic and can be found in fermented foods, yoghurt, cheese and supplements. It is used as probiotic because of its crucial properties like high tolerance to acid and bile, capability to adhere to intestinal surfaces, withstand low pH of gastric juices, inhibiting potentially pathogenic species, resisting antibiotics, producing exopolysaccharides and removing cholesterol. It is effective in preventing antibiotic-associated diarrhoea (Sabina, 2014).

Synbiotic refers to food ingredients or dietary supplements combining prebiotics and probiotics in a form of synergism, that beneficially affect the host by improving the survival and implantation of live microbial dietary supplements in the gastrointestinal tract, by selectively stimulating the growth and / or by activating the metabolism of one or a limited number of health promoting bacteria, thus improving host welfare.


Skim milk: Fresh whole milk was received from Livestock Farm Complex, Veterinary College and Research Institute, Namakkal. The skim milk was obtained from whole milk by centrifugation method after separation of cream.

Pectin: Neotea pectin powder was purchased from Neoteric DCBA Ideas, TN and was preserved in moisture proof pack for incorporation in cottage cheese.

Starter culture: Cheese starter culture and Lactobacillus acidophilus (LA-5) was procured in freeze dried form, from National Collection of Dairy cultures, NDRI, Karnal for the preparation of cottage cheese.

Preparation of synbiotic cottage cheese: Cottage cheese was manufactured using the short set method as described by Blanchette et al. (1996), with mild modification of procedure.

Sensory evaluation: The synbiotic cottage cheese was evaluated by the panelists on the basis of 9- point hedonic scale where 9 indicate extremely like and 1 extremely dislike (Amerine et al., 1965).

Physico-chemical parameters: The pH of cottage cheese estimated using a digital pH meter. The coagulation time was estimated by glass rod test.

Cheese yield:  The cheese yield was calculated as the cheese mass per equivalent mass of the initial milk (Ogunlade et al., 2017).

% of cheese yield = grams of cheese produced /grams of milk used x 100

Microbial analysis: The synbiotic cottage cheese and control samples were analyzed for different microbial parameters such as probiotic viable  count,coliform count and yeast and mould count by adopting standard procedure according to ICAR Dairy bacteriological manual (1972).

Statistical analysis: The data were analyzed by one way ANOVA in SPSS (version 20.0).


Sensory evaluation: Table 1 gives the score for sensory parameters, which includes colour and appearance, body and texture, flavour as well as the overall acceptability of synbiotic cottage cheese with various pectin level. The highest sensory evaluation presented for colour and appearance, body and texture, flavor and taste and overall acceptability is for synbiotic cottage cheese prepared by using 0.3 per cent pectin level.

Physico-chemical analysis: The prepared synbiotic cottage cheese with 0.3 per cent pectin was subjected to various physico-chemical analysis (pH, coagulation time and yield) and the results are presented in Table 2 and Table 3. The coagulation time and yield of the synbiotic cottage cheese using commercial cheese strain and Lactobacillus acidophilus (LA-5) has significance difference (P<0.05). The pH for both the treatment varies significantly both in between the treatment as well as between the storage period (P<0.05).

 Microbial analysis: The prepared synbiotic cottage cheese with 0.3 per cent pectin level was subjected to various microbial analysis and the results are presented in Table 4.

 The sensory values for the desired level of pectin almost resembles to the values obtained by Sadia et al. (2016) for cottage cheese made from cow milk. The result of yield of the two synbiotic cottage cheese are remarkably similar to that of Satterness et al. (1978) for culture method of making cottage cheese. The pH values are almost similar to those of Blanchette et al. (1996). The coliform count falls under the acceptable range of not more than 10 cfu/ml or 10 cfu/g of sample (FDA, 2015). Our results are as per the results obtained by Aylward et al. (1980). Also Trmcic et al. (2016) has observed that 16 % of fresh cheese used in the study were positive for coliforms at a level >10 cfu/g. The probiotic count markly resembles the results of Rafael et al. (2013), Araujo et al. (2010) and Gomes et al. (2011) and the viable counts are more than that of recommended probiotic viability in dairy foods. The present study is concluded that synbiotic cottage cheese incorporated with 0.3% pectin scored good overall acceptability and microbial quality with recommended levels of probiotic viability and it can cater the needs of present day health consciousness of consumer.


  1. Amerine, M. A., Pangborn, R. M., & Roessler, E. B. (1965). Principles of Sensory Evaluation of Food, Acad. Press, NY..
  2. Araújo, E. A., de Carvalho, A. F., Leandro, E. S., Furtado, M. M., & de Moraes, C. A. (2010). Development of a symbiotic cottage cheese added with Lactobacillus delbrueckii UFV H2b20 and inulin. Journal of Functional Foods2(1), 85-89.
  3. Aylward, E. B., O’Leary, J., & Langlois, B. E. (1980). Effect of milk storage on cottage cheese yield. Journal of Dairy Science63(11), 1819-1825.
  4. Blanchette, L., Roy, D., Belanger, G., & Gauthier, S. F. (1996). Production of cottage cheese using dressing fermented by bifidobaceria. Journal of dairy science79(1), 8-15.
  5. FDA (2015). “Standards for grade “A” milk and milk products,” in Grade “A” Pasteurized Milk Ordinance, (Washington, DC: U.S. Department of Health and Human Services, Public Health Service). 28–31
  6. Fijan, S. (2014). Microorganisms with claimed probiotic properties: an overview of recent literature. International journal of environmental research and public health11(5), 4745-4767.
  7. Gomes, A. A., Braga, S. P., Cruz, A. G., Cadena, R. S., Lollo, P. C. B., Carvalho, C., … & Bolini, H. M. A. (2011). Effect of the inoculation level of Lactobacillus acidophilus in probiotic cheese on the physicochemical features and sensory performance compared with commercial cheeses. Journal of Dairy Science94(10), 4777-4786.
  8. Hotchkiss Jr, A. T., Olano-Martin, E., Grace, W. E., Gibson, G. R., & Rastall, R. A. (2003). Pectic oligosaccharides as prebiotics.
  9. ICAR Microbiological testing of ice cream was determined as per the procedure given in, Manual in Dairy Bacteriology, 1972.
  10. Joint FAO/ WHO Working Group Report on drafting Guidelines for the Evaluation of Probiotics in Food, London, Ontario, Canada. April 30 – May 1, 2002.
  11. Oluwayemisi, O. A., Olusegun, O. V., & Okhonlaye, O. A. (2017). Percentage Yield and Proximate Composition of Cheese Produced from Sheep Milk Using Different Coagulants. International Journal of Microbiology and Biotechnology2(4), 171-175.
  12. Rasheed, S., Qazi, I. M., Ahmed, I., Durrani, Y., & Azmat, Z. (2016). Comparative study of cottage cheese prepared from various sources of milk. Proceedings of the Pakistan Academy of Sciences: Pakistan Academy of Sciences B. Life and Environmental Sciences53(4), 269-282.
  13. Satterness, D. E., Parsons, J. G., Martin, J. H., & Spurgeon, K. R. (1978). Yields of cottage cheese made with cultures and direct acidification. Cultured Dairy Products Journal (USA).
  14. Trmčić, A., Chauhan, K., Kent, D. J., Ralyea, R. D., Martin, N. H., Boor, K. J., & Wiedmann, M. (2016). Coliform detection in cheese is associated with specific cheese characteristics, but no association was found with pathogen detection. Journal of dairy science99(8), 6105-6120.
  15. Zamora-Vega, R., Montañez-Soto, J. L., Venegas-González, J., Bernardino-Nicanor, A., Cruz, L. G., & Martínez-Flores, H. E. (2013). Development and characterization of a symbiotic cheese added with Saccharomyces boulardii and inulin. African Journal of Microbiology Research7(23), 2828-2834.

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