ANTIFUNGAL ACTIVITIES AND CHEMICAL CHARACTERIZATION OF NEEM LEAF EXTRACTS ON THE GROWTH OF ALTERNARIA SPECIES – IN VITRO CULTURE MEDIUM
Department of Botany, D. A. V. (P.G.) College, Dehradun.
Received: 18-07-2015 Accepted: 24-10-2015
Azadirachta indica (Neem) leaf extract was taken to test its antifungal activity against three Alternaria spp. Ethanolic and Methanolic extracts in different concentrations (25%, 50%, 75% and 100%) was prepared and tested against test organisms using disc diffusion method. The crude aqueous and alcoholic leaf extracts of neem was more effective in inhibitions of growth of the fungi Alternaria alternata comparison to inhibitory effects on Alternaria solani growth in the artificial culture medium. Leaf extracts of neem which are cheap and environmentally safe are promising for protecting crop species against the fungal infestation and leading towards improvement of the crop in terms of yield and productivity. The assessment of fungi toxicity was carried out by food poison technique using four different extracts and their activity was recorded as radial growth and percentage inhibition. Ethanol and acetone extracts showed inhibition of growth of fungus; while methanol extract showed 50% inhibition and aqueous extract did not produce any inhibition of fungus. Findings from present study confirmed that ethanol and acetone extract of Azadirachta indica can be used as biofungicide to control this phytopathogenic fungus. Thus, the study ascertains the value of neem plant, which could be of considerable interest to the development of new antifungals.
Keywords: Antifungal, neem, Alternaria, Extract, Fungus