AMELIORATION BY SARPAGANDHA ROOT EXTRACT TO COUNTER DNA DAMAGE INDUCED BY CARBOFURAN IN FISH USING RAPD TOOL
Vidyanand Tiwari, Manoj Kumar and Sunil P. Trivedi*
Environmental Toxicology & Bioremediation Laboratory, Department of Zoology, University of Lucknow, Lucknow-226007, India
[Corresponding author E-mail*: email@example.com]
Received: 14-01-2016 Revised: 10-06-2016 Accepted: 12-06-2016
An assessment of ameliorative potential of Rauvolfia serpentina (Sarpagandha) root extract in fresh water food fish, Channa punctatus exposed to a carbamate pesticide, Carbofuran (CF) has been done for various haematological and biochemical parameters and in vivo genotoxicity in terms of RAPD. The fish specimens were exposed to sub-lethal concentration of CF (10% of LC50 i.e. 0.09 gl-1) in a semi-static system and the aforesaid parameters were assessed in exposed and control fish. The DNA damage was measured in blood cells by RAPD technique using oligo-nucleotide primers of fish specimens exposed to the sub-lethal concentration of CF. The primers viz., OPA-01, OPA-02, OPA-03, and OPA-13 were used in RAPD pattern assessment. Appearance or disappearance of bands and their intensities were evident in the RAPD pattern of fish specimens exposed to CF as compared to the control and the Sarpagandha root extract treated group. Alterations in biochemical and haematological parameters were observed in CF exposed fishes. However, these values showed that their levels tend to normalise on exposure to Sarpagandha root extract. This study explores the ameliorative effect of Sarpagandha that can be used for providing healthy environment to fish. Findings of present study have revealed the protective effect of Sarpagandha root extract against the carbofuran toxicity in fish Channa punctatus.
Keywords: ameliorative potential, Sarpagandha, Channa punctatus, primers, RAPD, haematology